Construction and characterization of a normalized cDNA library.
نویسندگان
چکیده
منابع مشابه
Construction of a normalized cDNA library by introduction of a semi-solid mRNA-cDNA hybridization system.
We report a novel procedure to construct a normalized (equalized) cDNA library. By introduction of the highly efficient self-hybridization system between a whole mRNA population and their corresponding cDNA immobilized on latex beads, which involves relatively simple manipulations, we were able to generate an mRNA population in which the copy number of abundant species was reduced while that of...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1994
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.91.20.9228